Journal Published Online: 01 May 1998
Volume 43, Issue 3

Maternal Identification from Skeletal Remains of an Infant Kept by the Alleged Mother for 16 Years with DNA Typing

CODEN: JFSCAS

Abstract

This is a case study concerning maternal identification by DNA typing at various loci. An infant skeleton was found in the alleged mother's apartment after it was kept for 16 years. We obtained the skeletal remains as well as saliva stains from the alleged mother. DNA typing was conducted for three loci in the HLA class II region (HLA-DQA1, -DPB1, and DRB1), five loci with the AmpliType PM kit (LDLR, GYPA, HBGG, D7S8, and GC), five STR loci (LPL, vWA, F13B, TH01, and TPOX) and D-loop region in mtDNA for maternal identification. Sex determination was accomplished using fluorescent DNA capillary electrophoresis typing. Approximately 5 ng of human DNA was recovered from 1 g of femur bone retrieved from the infant skeletal remains. The probability of two unrelated Japanese sharing the same genotypes was estimated as 7.2 × 10−11. The combined probability of exclusion that an individual is not the mother was also calculated at 0.998. We therefore conclude that the skeleton is from a female infant, and that there is no inconsistency in the claim that the infant was a daughter of the alleged mother.

Author Information

Yamamoto, T
Nagoya University School of Medicine, Nagoya, Japan
Uchihi, R
Nagoya University School of Medicine, Nagoya, Japan
Kojima, T
Nagoya University School of Medicine, Nagoya, Japan
Nozawa, H
Nagoya University School of Medicine, Nagoya, Japan
Huang, X-L
Nagoya University School of Medicine, Nagoya, Japan
Tamaki, K
Nagoya University School of Medicine, Nagoya, Japan
Katsumata, Y
Nagoya University School of Medicine, Nagoya, Japan
Pages: 5
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Stock #: JFS16221J
ISSN: 0022-1198
DOI: 10.1520/JFS16221J