Journal Published Online: 01 May 1998
Volume 43, Issue 3

Automated Extraction of Lysergic Acid Diethylamide (LSD) and N-demethyl-LSD from Blood, Serum, Plasma, and Urine Samples Using the Zymark RapidTrace™ with LC/MS/MS Confirmation

CODEN: JFSCAS

Abstract

A forensic procedure for the quantitative confirmation of lysergic acid diethylamide (LSD) and the qualitative confirmation of its metabolite, N-demethyl-LSD, in blood, serum, plasma, and urine samples is presented. The Zymark RapidTrace™ was used to perform fully automated solid-phase extractions of all specimen types. After extract evaporation, confirmations were performed using liquid chromatography (LC) followed by positive electrospray ionization (ESI+) mass spectrometry/mass spectrometry (MS/MS) without derivatization. Quantitation of LSD was accomplished using LSD-d3 as an internal standard. The limit of quantitation (LOQ) for LSD was 0.05 ng/mL. The limit of detection (LOD) for both LSD and N-demethyl-LSD was 0.025 ng/mL. The recovery of LSD was greater than 95% at levels of 0.1 ng/mL and 2.0 ng/mL. For LSD at 1.0 ng/mL, the within-run and between-run (different day) relative standard deviation (RSD) was 2.2% and 4.4%, respectively.

Author Information

de Kanel, J
National Medical Services, Inc., Willow Grove, PA
Vickery, WE
National Medical Services, Inc., Willow Grove, PA
Waldner, B
National Medical Services, Inc., Willow Grove, PA
Monahan, RM
National Medical Services, Inc., Willow Grove, PA
Diamond, FX
National Medical Services, Inc., Willow Grove, PA
Pages: 4
Price: $25.00
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Stock #: JFS16192J
ISSN: 0022-1198
DOI: 10.1520/JFS16192J