Journal Published Online: 01 March 1998
Volume 43, Issue 2

The DRPLA CAG Repeats in an Italian Population Sample: Evaluation of the Polymorphism for Forensic Applications

CODEN: JFSCAS

Abstract

The DRPLA CAG repeats polymorphism has been studied in an Italian population sample. PCR amplification, manual PAGE and silver staining were employed. A total of 16 different alleles, spanning the range from 5 to 21 CAG triplettes, was observed. The heterozygosity was 0.81 and no significant deviation from Hardy-Weinberg equilibrium was found. 81 meioses from parentage testing were also analyzed and a Mendelian pattern of inheritance was observed in all cases. In addition, we could successfully type DRPLA locus in some forensic specimens, 1 ng of DNA allowing clear definition of alleles. The authors conclude that the DRPLA CAG repeats analysis may be useful for forensic applications.

Author Information

Pelotti, S
Section of Legal Medicine, University of Bologna, Bologna, Italy
Mantovani, V
Malpighi Hospital Laboratory, Section of molecular and serological typing, Bologna, Italy
Esposti, PD
Section of Legal Medicine, University of Bologna, Bologna, Italy
D'Apote, L
Section of Legal Medicine, University of Bologna, Bologna, Italy
Bragliani, M
Malpighi Hospital Laboratory, Section of molecular and serological typing, Bologna, Italy
Maiolini, E
Section of Legal Medicine, University of Bologna, Bologna, Italy
Abbondanza, A
Section of Legal Medicine, University of Bologna, Bologna, Italy
Pappalardo, G
Section of Legal Medicine, University of Bologna, Bologna, Italy
Pages: 3
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Stock #: JFS16158J
ISSN: 0022-1198
DOI: 10.1520/JFS16158J