Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/d1s1676.htm. The volume of PCR reaction for each locus was 37.5 μL. The PCR products were analyzed by horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by silver staining (2). Data were analyzed using POWERSTATS program (3). The genotype distribution was analyzed for HardyWeinberg equilibrium according to Hou's method (4) and no deviation from Hardy-Weinberg equilibrium was observed.
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