Abstract

Samples were collected randomly from different individuals of East District of Sikkim. Nepali (N = 110). Bhutia ( N = 75) and Lepcha (N = 48) in form of either blood or buccal swab as per cooperation of participent. Genomic DNA was extracted by using standard phenol/chloroform procedure (2). Quantitation of DNA was carried out using the Quantiblot kit (PE Applied Biosystems) and subsequent PCR amplification was performed using the Powerplex 16 multiplex System (Promega Coip, Madison, U.S.A.) The products were detected on a 5% denaturing polyacrylamide sequencing gel using the ABI Prism 377 DNA Sequencer (PE Applied Biosystems) and genotype classification was made by comparison with allelic ladders provided with the Powerplex 16 System.

Author Information

Kashyap, VK
Central Forensic Science Laboratorq, Kolkata, West Bengal, India
Guha, S
Central Forensic Science Laboratorq, Kolkata, West Bengal, India
Trivedi, R
Central Forensic Science Laboratorq, Kolkata, West Bengal, India
Pages: 5
Price: $25.00
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Stock #: JFS15547J
ISSN: 0022-1198
DOI: 10.1520/JFS15547J