Blood samples (n = 224) from healthy unrelated individuals (males) were collected. DNA was extracted by using the phenolchloroform-isoamyl alcohol method (1). Hot-start PCR triplex (Triplex I: DYS19 and DYS389 I/II; Triplex II: DYS390, DYS391 and DYS393) and singleplex (DYS392) amplifications were accomplished with fluorescein labelled primers by the method published by Gené et al. (2). The primers used were those described by Kayser et al. (3) and de Knijff et al. (4). DYS385 locus was analyzed following the method and primers described by EDNAP group (5). Genotypes were analyzed in denaturing 6% polyacrilamide gel electrophoresis, using a monochrome automated laser fluorescence sequencer. The alleles from all loci reported were typed according to the published nomenclatures and the ISFG guidelines for STR analyses. Our group have received the certify of excellence in the Y-STR Haplotyping Quality Assurance Exercise 2000–2001 (http:/www.ystr.org).
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