Allele and genotype frequencies of the five STR loci (D10S2325, D13S325, D20S470, D18S51, and D19S253) were determined. Specimens were collected from unrelated volunteer blood donors from a population of Chungnam Province in South Korea (n = 130). DNA was obtained from the blood specimens using the Wizard genomic DNA purification kit (Promega, USA) and amplified using the multiplex PCR (triplex amplification of D10S2325, D13S325, and D20S470; duplex amplification of D18S51 and D19S253). The amplified DNAs were separated by denaturing polyacrylamide gel electrophoresis and then visualized by the silver staining (1). Exact x2-test for Hardy-Weinberg equilibrium and pairwise disequilibrium were analyzed using the GDA program written by Lewis P.O. and Zaykin D (2). The chance of exclusion of combined system was 0.9986.
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