Journal Published Online: 01 September 2002
Volume 47, Issue 5

Successful DNA Typing of a Urine Sample in a Doping Control Case Using Human Mitochondrial DNA Analysis

CODEN: JFSCAS

Abstract

In a doping control case, a urine sample was tested positive for nandrolon. We were asked by the athlete to perform DNA investigations on the questioned urine sample and compare these to a fresh blood sample taken from the athlete in order to detect or rule out manipulation and/or switching of the samples. The urine sample had been collected nine months prior to the investigation and had been stored at 4°C. In a first approach, nuclear DNA systems were investigated that failed with the exception of the Amelogenin system. Due to the high copy number of mitochondrial DNA molecules and the robustness of the mitochondrial genome, we investigated the HVR I and HVR II regions of mitochondrial DNA and obtained reproducible and clear sequencing results for both the blood and the urine samples. Due to the identical sequences, it could not be excluded that the blood sample and the urine sample were from the same individual or an individual having the same maternal lineage.

Author Information

Junge, A
Institute of Legal Medicine Rheinische Friedrich-Wilhelms-University Bonn, Bonn, Germany
Steevens, M
Institute of Legal Medicine Rheinische Friedrich-Wilhelms-University Bonn, Bonn, Germany
Madea, B
Institute of Legal Medicine Rheinische Friedrich-Wilhelms-University Bonn, Bonn, Germany
Pages: 3
Price: $25.00
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Stock #: JFS15509J
ISSN: 0022-1198
DOI: 10.1520/JFS15509J