Blood samples were obtained from unrelated male individuals from the four population groups and placed on FTA® paper (Fitzco-Whatman). DNA was extracted using the aqueous processing method for FTA® paper. One extracted disc (1.2 mm diameter) was used in the PCR per sample. The mixed male DNA sample was loaded onto a pre-washed FTA® paper and subjected to an alkalineisopropanol treatment. A combination of three Y-linked microsatellites loci, DYS438 (1), DYS390 (2), and DYS439 (1) and amelogenin were amplified simultaneously using a fluorescent multiplex system. DYS438 and amelogenin were labelled with 6-FAM, DYS390 with JOE and DYS439 with TAMRA.

Author Information

Chang, YM
Forensic Science Centre, Adelaide, SA, Australia
Both, K
Forensic Science Centre, Adelaide, SA, Australia
Burgoyne, LA
School of Biological Sciences, Flinders University of South Australia, Bedford Park, SA, Australia
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Stock #: JFS15483J
ISSN: 0022-1198
DOI: 10.1520/JFS15483J