Genomic DNA was obtained from blood samples by using standard phenol/chloroform procedure (2). Quantitation of DNA was carried out using the Quantiblot kit (PE Applied Biosystems) and subsequent PCR amplification was performed using the Powerplex ™ 16 System (Promega Corp.) The products were detected on a 5% denaturing polyacrylamide sequencing gels using the ABI Prism™ 377 DNA Sequencer (PE Applied Biosystems) and genotype classification was made by comparison with allelic ladders provided with the Powerplex™ 16 System.
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