Journal Published Online: 01 July 2002
Volume 47, Issue 4

HLA-DQA1 and Polymarker Loci Allele Frequencies in the Bharias, a Primitive Indian Tribal Population from Patalkot, India

CODEN: JFSCAS

Abstract

Twenty-three specimens were collected from unrelated volunteer blood donors. DNA was obtained from blood specimens using “QIAamp Blood and Tissue kit” (QIAGEN GmbH, Hilden, Germany) according to the manufacturer's recommended protocol. Amplification was carried out in a “Gene Amp® PCR system 2400” thermal cycler (Perkin Elmer Corporation, California) using 5 ng target DNA. Typing of the six loci was performed by reverse dot blot with Allele Specific Oligonucleotides (ASO) probes, using “AmpliType® PMHLADQA1 PCR amplification and typing kit” (Roche Molecular Systems, Inc., New Jersey).

Author Information

Biswas, R
Central Forensic Science Laboratory, Chandigarh, India
Giroti, R
Central Forensic Science Laboratory, Chandigarh, India
Pages: 2
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Stock #: JFS15481J
ISSN: 0022-1198
DOI: 10.1520/JFS15481J