The clarity of VNTR allele profiles generated by vertical polyacrylamide electrophoresis is improved by the addition of a stacking gel layer. However, the size of the resolving gel must be reduced to provide space for the stacking gel; therefore, resolving power is conceded in order to improve clarity. The procedure described in this paper delivers near maximum allele separation while maintaining excellent band clarity. This has been achieved by reducing the length of the stacking gel and dividing the resolving gel into two layers with a small increment in acrylamide in the lower layer.
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