Journal Published Online: 01 March 2001
Volume 47, Issue 2

Y-Chromosome STR Haplotypes in Central-West African Immigrant in Spain Population Sample

CODEN: JFSCAS

Abstract

Blood samples were obtained from 103 healthy unrelated black West African immigrant population in Spain from different countries situated to the south of the Sahara desert. The DNA was extracted using Chelex 100 protocol as described by Walsh et al. (1). The quantity of recovered DNA was determined using QuantiBlot® Human DNA Quantitation Kit (Perkin Elmer). DYS19, DYS389I, DYS389II, DYS390, and DYS393 were amplified as described by Gusmao et al. (2). The DYS385 amplification conditions complied with the methodology described by Schneider et al. (3). The PCR was performed in a Perkin Elmer 9600 thermocycler.

Author Information

Gamero, J-J
University of Cadiz, Cadiz, Spain
Romero, J-L
University of Cadiz, Cadiz, Spain
Gonzalez, J-L
University of Cadiz, Cadiz, Spain
Carvalho, M
Institute of Legal Medicine of Coimbra, Coimbra, Portugal
Anjos, M-J
Institute of Legal Medicine of Coimbra, Coimbra, Portugal
Real, FC
Institute of Legal Medicine of Coimbra, Coimbra, Portugal
Vieira, D-N
Institute of Legal Medicine of Coimbra, Coimbra, Portugal
Vide, M-C
Institute of Legal Medicine of Coimbra, Coimbra, Portugal
Pages: 3
Price: $25.00
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Stock #: JFS15277J
ISSN: 0022-1198
DOI: 10.1520/JFS15277J