Journal Published Online: 01 March 2001
Volume 47, Issue 2

Allele Frequencies for Eight STR Loci in a Population Sample from Sicily (Italy)

CODEN: JFSCAS

Abstract

Blood samples from healthy and unrelated individuals (n=135–150) were extracted by Chelex 100 procedure (1). DNA samples (5–25 ng) were amplified in a “PCR sprint” (Hybaid) thermal cycler and typed-classified according to the DNA commission of the ISFH (2). Allele and genotype frequencies were determined and Hardy-Weinberg equilibrium was tested by aX2 method. From the genotype data, the power of discrimination (PD) according to the equation suggested by Fisher (3) was calculated. The heterozigosity value (OH) as described by Nei and Roychoudhury (4) was determined.

Author Information

Asmundo, A
Dipartimento di Medicina Sociale del Territorio (D.M.S.T.), Sezione di Medicina Legale; Universita degli Studi di Messina, Italy
Sapienza, D
Dipartimento di Medicina Sociale del Territorio (D.M.S.T.), Sezione di Medicina del Lavoro; Universita degli Studi di Messina, Italy
Crino, C
Dipartimento di Medicina Sociale del Territorio (D.M.S.T.), Sezione di Medicina Legale; Universita degli Studi di Messina, Italy
Pages: 2
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Stock #: JFS15271J
ISSN: 0022-1198
DOI: 10.1520/JFS15271J