Journal Published Online: 01 March 2002
Volume 47, Issue 2

Determination of ABO Blood Grouping from Human Oral Squamous Epithelium by the Highly Sensitive Immunohistochemical Staining Method EnVision+

CODEN: JFSCAS

Abstract

Using the highly sensitive immunohistochemical staining method EnVision+, which employs a dextran polymer reagent for the secondary antibody, the detection of the ABH antigens was attempted in the oral squamous epithelium. This new technique uses monoclonal antibody as a primary antibody and it takes about three hours for staining. The time is much shorter than conventional absorption-elution testing or absorption-inhibition testing for the determination of ABO blood grouping. Secretor saliva samples were stained at strong intensity by the antibody, which corresponded to its blood group and anti-H. On the one hand, nonsecretor saliva samples were stained at strong intensity only by the antibody that corresponded to its blood group, and at weak intensity only by anti-H. Since human oral squamous epithelium antigens were stained specifically by this method, we can examine the ABO blood group of saliva samples and perform cytodiagnosis at the same time.

Our research suggested that the EnVision+ Method is a useful technique for ABO blood grouping of saliva in forensic cases.

Author Information

Noda, H
Kinki University School of Medicine, Osaka, Japan
Yokota, M
Kinki University School of Medicine, Osaka, Japan
Tatsumi, S
Kinki University School of Medicine, Osaka, Japan
Sugiyama, S
Kinki University School of Medicine, Osaka, Japan
Pages: 4
Price: $25.00
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Stock #: JFS15254J
ISSN: 0022-1198
DOI: 10.1520/JFS15254J