Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Chengdu of China. DNA was extracted using Chelex method, (1). PCR amplification conditions can be accessed at http://www.legalmed.org/dna/d1d2d17.htm. The PCR reaction volume for each locus was 37.5 µL. The PCR products were analyzed by horizontal nondenaturing polyacrylamide gel electrophoresis with discontinuous buffer system and visualized by sliver staining (2). Data of population genetics and forensic science were analyzed using POWERSTATS program (3). The genotype distribution was analyzed for Hardy-Weinberg equilibrium according to Hou's method (4), no deviation from Hardy-Weinberg equilibrium was observed.
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