Journal Published Online: 01 September 2001
Volume 46, Issue 5

The Use of the D-, L- Aspartic Ratio in Decalcified Collagen from Human Dentin as an Estimator of Human Age



Among the methods dealing with the age estimation, the evaluation of the ratio of the D-, L- form of the aspartic acid in tissues with a low metabolic turnover is considered to be the most precise. We introduced demineralization of the dentin with 0.5 M EDTA adjusted to pH = 7.4. The advantage of such a procedure is that after demineralization we obtained pure insoluble protein (collagen) and soluble noncollagenous proteins in one step. In this study we analyzed insoluble collagen. The amino acids obtained after the hydrolysis were derivatized into TFA isopropyl esters and analyzed by gas chromatography on Chirasil L-Val capillary column. We analyzed human dentin from the lower canines. The correlation coefficient was 0.93 for our set of 71 persons. The result concurred with those of other scientists.

Author Information

Pilin, A
Institute of Forensic Medicine and Toxicology, University Hospital in Prague, Czech Republic
Čabala, R
Faculty of Science, Charles University, Prague, Hlavova 2030, Czech Republic
Pudil, F
Institute of Chemical Technology, Czech Republic
Bencko, V
Institute of Hygiene and Epidemiology of the 1st Medical Faculty, Charles University, Prague, Czech Republic
Pages: 4
Price: $25.00
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Stock #: JFS15126J
ISSN: 0022-1198
DOI: 10.1520/JFS15126J