Journal Published Online: 01 March 2001
Volume 46, Issue 2

The Application of Minisatellite Variant Repeat Mapping by PCR (MVR-PCR) in a Paternity Case Showing False Exclusion Due to STR Mutation

CODEN: JFSCAS

Abstract

A boy and a girl with their mother brought a paternity suit against an alleged but deceased father. We tested six conventional genetic markers, the AmpliType PM+DQA1 and twelve STR loci the children and mother together with the alleged paternal grandparents. We also DNA typed the bloodstain found later in the alleged father's medical record. Only the result at D3S1358 in a nineplex STR system excluded the alleged father from parentage of the boy, whereas all markers were inclusive for the girl. Accordingly, we performed sequence analysis at D3S1358 to confirm the presence of a paternal exclusion or mutation. The sequence analysis indicated that the boy's allele 17 could have originated from either of the alleged father's allele 16 or 18 by a single-step mutation associated with slippage mutation in STR loci. We carried out minisatellite variant repeat mapping by PCR (MVR-PCR) at loci D1S8 (MS32) and D7S21 (MS31A) and mapped allele haplotypes of all individuals except the deceased alleged father. The MVR-PCR analysis showed that the boy has no inconsistency with the relationship between the alleged grandparents, and was very effective at increasing the paternity index (PI) value. We conclude that there is biological relationship between not only the girl but also the boy and the alleged father.

Author Information

Yamamoto, T
Postgraduate School of Medicine, Nagoya University, Nagoya, Japan
Tamaki, K
Sapporo Medical University School of Medicine, Chuo-ku, Sapporo, Japan
Huang, X-L
Tokai University School of Medicine, Bohseidai, Isehara, Japan
Yoshimoto, T
Postgraduate School of Medicine, Nagoya University, Nagoya, Japan
Mizutani, M
Postgraduate School of Medicine, Nagoya University, Nagoya, Japan
Uchihi, R
Postgraduate School of Medicine, Nagoya University, Nagoya, Japan
Katsumata, Y
Postgraduate School of Medicine, Nagoya University, Nagoya, Japan
Jeffreys, AJ
University of Leicester, Leicester, U.K.
Pages: 5
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Stock #: JFS14976J
ISSN: 0022-1198
DOI: 10.1520/JFS14976J