Journal Published Online: 01 March 2001
Volume 46, Issue 2

Enhancement of an Insufficient Dye-Formation in the Ninhydrin Reaction by a Suitable Post Treatment Process



A study of the reaction between ninhydrin and alanine has been carried out taking into account that, adhered on the surface of a dry porous medium such as paper, a quasi-heterogeneous reaction has to take place. Instead of amino acids released from human sweat glands, aqueous solutions of alanine were taken to deliver a given amount of amino acid to the sample. The dye density, obtained after using a standard development process, could noticeably be increased by setting a drop of water on the dye dot, thus indicating that not all the alanine had been used for dye formation during the usually applied process. The incomplete reaction can be explained by the problem of bringing the reactants into contact with each other when both are in the solid phase in the porous surrounding. The temporary presence of water allows a reorientation of the insoluble reactants. With fingerprints an increase in both the rate of development and the final dye density could be obtained when the sample was post treated after the developing process with the blank solvent, thus also the background coloration could be decreased. The ideas presented in this paper may form the basis for a modification of developing processes with ninhydrin in order to increase the proportion of amino acids present (in the sample) used in dye formation without data loss.

Author Information

Ramminger, U
Institute of Physical and Theoretical Chemistry, University of Erlangen-Nuremberg, Germany
Nickel, U
Institute of Physical and Theoretical Chemistry, University of Erlangen-Nuremberg, Germany
Geide, B
Federal Bureau of Criminal Investigation, Wiesbaden, Germany
Pages: 6
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Stock #: JFS14961J
ISSN: 0022-1198
DOI: 10.1520/JFS14961J