Journal Published Online: 01 January 2001
Volume 46, Issue 1

A Silica-Based Mitochondrial DNA Extraction Method Applied to Forensic Hair Shafts and Teeth



The purpose of this study is to evaluate the applicability of a nonorganic DNA extraction method for use in the analysis of environmentally compromised forensic hair shaft and tooth samples. The condition of the samples included cases of water decomposition, severe incineration, and varying stages of putrefaction. Enzymatic amplification and manual sequencing of the first segment of the mitochondrial hypervariable region were performed successfully on each of the 20 autopsied individuals. The results indicate that the silica-based extraction method produces mtDNA suitable for genetic identification from forensic samples including hair shafts and teeth.

Author Information

Baker, LE
The University of Tennessee, Knoxville, Knoxville, TN
McCormick, WF
The University of Tennessee, Knoxville, Knoxville, TN
Matteson, KJ
University of Tennessee Medical Center, Knoxville, TN
Pages: 5
Price: $25.00
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Stock #: JFS14923J
ISSN: 0022-1198
DOI: 10.1520/JFS14923J