Journal Published Online: 01 November 2000
Volume 45, Issue 6

Detection and Correction of a Migration Anomaly on a 310 Genetic Analyzer



During STR analysis on the 310 Genetic Analyzer, retarded migration of GS500ROX size standards and alleles in some samples was observed. The contribution of reagents, capillary and performance optimized polymer POP 4 to the observed anomaly was experimentally eliminated. Variation in electrophoresis temperature between 55°C and 65°C did not alter the rate of migration of GX500ROX size standard and sample alleles. An eroded connector for the cathode mounted on the heat plate assembly caused the abnormal migration. Hence, it is important to verify the mobility of all fragments in the size standard for each sample to avoid any erroneous allele calls by the automated data analysis software.

Author Information

Shewale, JG
ReliaGene Technologies, Inc., New Orleans, LA
Richey, SL
ReliaGene Technologies, Inc., New Orleans, LA
Sinha, SK
ReliaGene Technologies, Inc., New Orleans, LA
Pages: 4
Price: $25.00
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Stock #: JFS14893J
ISSN: 0022-1198
DOI: 10.1520/JFS14893J