Journal Published Online: 01 July 2000
Volume 45, Issue 4

Gelatinase A in Human Dentin as a New Biochemical Marker for Age Estimation



Dentin proteins from 24 individual permanent molars from patients aged 15 to 73 years were sequentially extracted, with guanidinium chloride (G1-extract) and then with EDTA; after demineralization, the material was again extracted with guanidinium chloride (G2-extract). Extracts were analyzed by SDS-PAGE electrophoresis and the gels were processed for zymography to detect gelatinolytic activities. The patterns of gelatinase A distribution differed in the different dentin protein fractions, and the changes varied with age. Significant differences were detected in gelatinase A in G2-extracts between individual younger than 20 years old and the rest of the sample (xexp2 ,429; 1 d.f.; p < 0.001). The incidence of true and false positives and negatives, and sensitivity and specificity for the presence of gelatinase A in dentin extracts, were calculated. Determination of gelatinase A in human dentin may be a useful marker to estimate age, especially when other morphological methods are of limited usefulness.

Author Information

Martín-de las Heras, S
Faculty of Medicine University of Granada, Granada, Spain
Valenzuela, A
Faculty of Medicine University of Granada, Granada, Spain
Overall, CM
Faculty of Dentistry, University of British Columbia, Vancouver, Canada
Pages: 5
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Stock #: JFS14774J
ISSN: 0022-1198
DOI: 10.1520/JFS14774J