Journal Published Online: 01 May 2000
Volume 45, Issue 3

Population Studies and Validation of Paternity Determinations by Six Microsatellite Loci

CODEN: JFSCAS

Abstract

A single locus system of 6 microsatellite markers was evaluated for paternity testing. A nonradioactive method based on peroxidase labeling of a DNA probe was used to estimate the allele frequency of markers D1S216, D3S1217, D7S480, D9S157, D13S153, and D16S422 by genotyping 1134–1698 chromosomes. The number of detected alleles were 22, 15, 23, 10, 16, and 19, respectively, and the allele frequency varied from 0.001 to 0.317. The genotype of 87 families, consisting of mother, father, and child was determined. The probability that a random individual will give a positive paternity was evaluated. We conclude that the markers can be reliably typed and give sufficient and reliable information for paternity testing.

Author Information

Ingvarsson, S
University Hospital of Iceland, Reykjavik, Iceland
Finnsdottir, V
University Hospital of Iceland, Reykjavik, Iceland
Sigurdsson, A
University Hospital of Iceland, Reykjavik, Iceland
Geirsson, G
University Hospital of Iceland, Reykjavik, Iceland
Pages: 4
Price: $25.00
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Stock #: JFS14751J
ISSN: 0022-1198
DOI: 10.1520/JFS14751J