Blood samples were collected in EDTA coated vacutainers from 207 unrelated patients who visited the King Khaled University Hospital and Security Force Hospital in Riyadh, Saudi Arabia. Blood saturated cotton swatches were prepared from these samples and dried at room temperature. DNA was extracted from the swatches by organic extraction (phenol/chloroform/isoamyl alcohol) followed by ethanol precipitation (1). The quantity of DNA was determined using the Quantiblot kit.
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