Journal Published Online: 01 January 2000
Volume 45, Issue 1

Determination of Amphetamine by HPLC After Acetylation



An analytical procedure has been developed for the HPLC determination of amphetamine by off-line pre-column derivatization. The proposed procedure consists of sample preparation by acetylation of amphetamine with acetic anhydride and a subsequent reversed-phase HPLC separation on an octadecyl silica stationary phase with salt-free mobile phase (tetrahydrofuran, acetonitrile, 0.1% triethylamine in water, 15:15:70 v/v) applying UV-detection. The applicability of the elaborated procedure is demonstrated with results obtained by analysis of real samples seized in the Hungarian black market.

Author Information

Veress, T
Institute for Forensic Sciences, Budapest, Hungary
Pages: 6
Price: $25.00
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Stock #: JFS14652J
ISSN: 0022-1198
DOI: 10.1520/JFS14652J