Journal Published Online: 01 July 1999
Volume 44, Issue 4

Simultaneous Detection of Multiple STR Loci on Sex Chromosomes for Forensic Testing of Sex and Identity

CODEN: JFSCAS

Abstract

The forensic usefulness of X and Y chromosomal STR loci has recently been demonstrated. One quadruplex-PCR, using 2 X- and 2 Y-STRs (STRX1/HPRTB and DYS390/DYS393), and 2 duplex-PCRs, each using an X- and a Y-STR (ARA/DYS390 and ARA/DYS393), and detection of PCR products by using an automated DNA sequencer are reported herein. This approach allows us to determine not only the sex of the donor of a sample, but also the X- and/or Y-STR genotypes of the sample. A male biological specimen yields 4 amplified products in quadruplex-PCR and 2 amplified fragments in duplex-PCRs, whereas a female biological specimen yields only 2 amplified fragments of X-STR in quadruplex-PCR and one fragment, also of X-STR, in duplex-PCRs. Our study thus provides useful information for many activities in forensic practice, such as identity testing, paternity testing, especially of deficiency cases, compilation of population data, and sex determination of a biological sample from a single PCR.

Author Information

Tun, Z
Osaka University Graduate School of Medical F3, Suita Osaka, Japan
Honda, K
Osaka University Graduate School of Medical, Osaka, Japan
Nakatome, M
Osaka University Graduate School of Medical, Osaka, Japan
Nakamura, M
Osaka University Graduate School of Medical, Osaka, Japan
Shimada, S
Osaka University Graduate School of Medical, Osaka, Japan
Ogura, Y
Osaka University Graduate School of Medical, Osaka, Japan
Kuroki, H
Osaka University Graduate School of Medical, Osaka, Japan
Yamazaki, M
Osaka University Graduate School of Medical, Osaka, Japan
Terada, M
Osaka University Graduate School of Medical, Osaka, Japan
Matoba, R
Osaka University Graduate School of Medical, Osaka, Japan
Pages: 6
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Stock #: JFS14552J
ISSN: 0022-1198
DOI: 10.1520/JFS14552J