Journal Published Online: 01 March 1999
Volume 44, Issue 2

The Determination of Lysergide (LSD) in Urine by High-Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry (IDMS)



The use of isotope dilution mass spectrometry (IDMS) has been investigated for the forensic confirmation of lysergic acid diethylamide (LSD) in urine by LC-MS. The advantages of using a deuterated analog of LSD as an internal standard over methysergide are discussed. This study includes a comparison of the electrospray mass spectra of LSD, LSD-d3 and methysergide, and discusses the choice of suitable ions for use in selected ion monitoring (SIM) mode. An IDMS method is presented for the LC-MS confirmation of LSD in urine, with a limit of quantification (LOQ) of 0.5 ng/mL, reflecting the forensic requirement at this laboratory. Under some circumstances the LOQ can be improved to 0.1 ng/mL. This method is linear in the range tested (up to 10 ng/mL LSD in urine) and has been validated in terms of accuracy and precision.

Author Information

White, SA
Laboratory of the Government Chemist, Teddington Middlesex, TW, England
Kidd, AS
Biomedical Science Division, Kings College London, Strand, London, England
Webb, KS
Laboratory of the Government Chemist, Teddington Middlesex, TW, England
Pages: 5
Price: $25.00
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Stock #: JFS14467J
ISSN: 0022-1198
DOI: 10.1520/JFS14467J