Journal Published Online: 01 January 1999
Volume 44, Issue 1

Deoxyribonuclease I Phenotyping from Saliva Stains

CODEN: JFSCAS

Abstract

Good typing results were obtained using a newly developed method for extraction and purification of deoxyribonuclease I (DNase I) from saliva stains. Previously, DNase I phenotyping from saliva stains has been unsuccessful because of low enzyme activity and heavy contamination. Salivary DNase I was extracted from stains using phosphate buffer containing Nonidet P-40. Extracts were purified using Phenyl Sepharose CL-4B gel. Electrophoresis was performed, and DNase I was successfully phenotyped. All of the DNase I phenotypes, which were obtained from saliva stains using this new method, were identical to the phenotypes determined from urine samples. Moreover, DNase I was correctly phenotyped from saliva stains that had been stored for over three months at room temperature or at 37°C. These results suggest that DNase I polymorphisms provide valuable information for forensic characterization of saliva stains.

Author Information

Iida, R
Fukui Medical University, Fukui, Japan
Sawazaki, K
Fukui Medical University, Fukui, Japan Scientific Investigation Laboratory, Fukui Prefectural Police Headquarters, Fukui, Japan
Yasuda, T
Gunma University School of Medicine, Gunma, Japan
Tsubota, E
Fukui Medical University, Fukui, Japan
Aoyama, M
Fukui Medical University, Fukui, Japan
Matsuki, T
Fukui Medical University, Fukui, Japan
Kishi, K
Gunma University School of Medicine, Gunma, Japan
Pages: 4
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Stock #: JFS14428J
ISSN: 0022-1198
DOI: 10.1520/JFS14428J