Journal Published Online: 01 January 1999
Volume 44, Issue 1

Blood Grouping of Mixed Bloodstains Using Immunocytochemical Methods

CODEN: JFSCAS

Abstract

Immunocytochemical methods to determine the ABO blood group of each blood of mixed bloodstains have been developed. Mixed bloodstains were made on surgical blades and a cedar board. The blades were dipped into blood and then dipped into blood of a different group at intervals of 30, 20, 15, 10 and 5 s. Two drops of blood were dropped on a cedar board and then two drops of blood of a different group were dropped there at the same intervals. The bloodstains were dried for a week. The blood samples were removed from the blades or the cedar board and processed according with a routine histological method. Three serial thin sections were obtained. After deparaffinization, the sections were treated in papain solution for 2 h at 36°C, to unmask antigenic sites on red cell membranes. The labeled streptavidin-biotin (LSAB) and peroxidase-anti-peroxidase (PAP) methods were used to detect A and B antigens, and an indirect immunocytochemical method for H antigen. These immunocytochemical methods showed specific immunologic reactions and allowed determination of the blood group of each blood of mixed bloodstains. Further, these methods indicated a possibility to determine who was stabbed first, in cases where two or more victims were stabbed with a single knife.

Author Information

Bunai, Y
Gifu University School of Medicine, Gifu, Japan
Nakamura, I
Gifu University School of Medicine, Gifu, Japan
Nagai, A
Gifu University School of Medicine, Gifu, Japan
Yamada, S
Gifu University School of Medicine, Gifu, Japan
Watanabe, Y
Gifu University School of Medicine, Gifu, Japan
Takayama, T
Gifu University School of Medicine, Gifu, Japan
Ohya, I
Gifu University School of Medicine, Gifu, Japan
Pages: 5
Price: $25.00
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Stock #: JFS14418J
ISSN: 0022-1198
DOI: 10.1520/JFS14418J