Journal Published Online: 01 July 1997
Volume 42, Issue 4

The Applicability of Formalin-Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis



Historically, formalin fixed (FF) tissues could not be used as a source of DNA in forensic science due to the fact that the DNA was too degraded for DNA analysis. With the introduction of the polymerase chain reaction (PCR) technique to forensic science, the usefulness of DNA from this biological material has been re-evaluated. This study evaluates the potential use of DNA from FF and formalin fixed paraffin embedded (FFPE) tissues in 13 PCR systems; HLA DQα, LDLR, GYPA, HBGG, D7S8, GC, D1S80, vWA31, THO1, F13A1, FES/FPS, TPOX, and CSF1PO. The first six, HLA DQα, LDLR, GYPA, HBGG, D7S8, and GC are reverse dot blot systems, D1S80 is an amplified fragment length polymorphism (AmpFlp) system and the others are short tandem repeats (STRs). This study shows that FFPE tissue which has not been fixed in formalin for more than three days is a useful source of DNA for 12 of the 13 PCR systems. In contrast, FF tissue did not prove to be a reliable source of DNA for the PCR techniques examined here.

Author Information

Romero, RL
Washoe County Sheriff's Office, Forensic Science Division, Reno, NV
Juston, AC
Suffolk County Crime Laboratory, North County Complex, Hauppauge, NY
Ballantyne, J
Suffolk County Crime Laboratory, North County Complex, Hauppauge, NY
Henry, BE
Alabama Department of Forensic Science, Birmingham, AL
Pages: 7
Price: $25.00
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Stock #: JFS14188J
ISSN: 0022-1198
DOI: 10.1520/JFS14188J