Journal Published Online: 01 July 1997
Volume 42, Issue 4

Sequence Analysis of Domestic Dog Mitochondrial DNA for Forensic Use



A method has been developed for the direct sequencing of hypervariable region 1 (HVI) of domestic dog (Canis familiaris) and wolf (Canis lupus) mitochondrial DNA (mtDNA) using single hairs as template. The method uses a robotic work-station and an automated sequencer to allow for robust routine analysis. A population data base was created in order to investigate the forensic and population-genetic informativeness of domestic dog HVI. Sequence variation, partitioning of dog breeds among sequence variants and phylogenetic relations between the variants were determined. Samples from 102 domestic dogs of 52 different breeds and two captive wolves were analyzed. Nineteen dog-sequence variants were found and the frequencies of the variants ranged from 1 to 21%. The calculated discrimination power of the region, i.e., the exclusion capacity, implied that nine out of ten disputed individuals can be excluded by this analysis. The sequence variants were found to cluster into four phylogenetic groups.

Author Information

Savolainen, P
Royal Institute of Technology, Stockholm, Sweden
Rosén, B
The National Laboratory of Forensic Science, Linköping, Sweden
Holmberg, A
Royal Institute of Technology, Stockholm, Sweden
Leitner, T
Royal Institute of Technology, Stockholm, Sweden Swedish Institute for Infectious Disease Control, Stockholm, Sweden
Uhlén, M
Royal Institute of Technology, Stockholm, Sweden
Lundeberg, J
Royal Institute of Technology, Stockholm, Sweden
Pages: 8
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Stock #: JFS14170J
ISSN: 0022-1198
DOI: 10.1520/JFS14170J