Journal Published Online: 01 November 1996
Volume 41, Issue 6

PCR Analysis of Lewis-Negative Gene Mutations and the Distribution of Lewis Alleles in a Japanese Population

CODEN: JFSCAS

Abstract

Three mutations in the Lewis-negative gene, T59G, G508A and T1067A, have been detected by means of a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 149 unrelated Japanese individuals. We found three common Lewis alleles—Le (without the T59G, G508A, and T1067A mutations), le1 (with the T59G and G508A mutations), and le2 (with the T59G and T1067A mutations) in a Japanese population. In addition, we also found one rare Lewis-negative allele, le3 (only with the T1067A mutation). The allele frequencies of Le, le1, le2, and le3 were 0.607, 0.275, 0.114, and 0.003, respectively. Our results were in accordance with those expected by the Hardy-Weinberg equilibrium. Some statistical parameters of forensic interest were also calculated.

Author Information

Liu, Y
Kurume University School of Medicine, Kurume, Fukuoka, Japan
Koda, Y
Kurume University School of Medicine, Kurume, Fukuoka, Japan
Soejima, M
Kurume University School of Medicine, Kurume, Fukuoka, Japan
Uchida, N
Kurume University School of Medicine, Kurume, Fukuoka, Japan
Kimura, H
Kurume University School of Medicine, Kurume, Fukuoka, Japan
Pages: 4
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Stock #: JFS14040J
ISSN: 0022-1198
DOI: 10.1520/JFS14040J