Journal Published Online: 01 September 1996
Volume 41, Issue 5

A New Individualization Marker of Sweat: Deoxyribonuclease I (DNase I) Polymorphism

CODEN: JFSCAS

Abstract

We have confirmed for the first time, both biochemically and immunologically, the existence of deoxyribonuclease I (DNase I) in human liquid sweat. Isoelectric focusing of sweat samples on polyacrylamide gels (pH 3.5 to 5), followed by dried agarose film overlay detection, was used to determine the phenotypes of sweat DNase I. Because this detection method not only had high sensitivity, but also high band resolution, it was possible to determine DNase I types from sweat samples of 50 to 100 μL. Pretreatment of sweat samples with sialidase was essential for typing to enhance markedly the sensitivity accompanied by simplification of the isozyme pattern. The DNase I types in all sweat samples were consistently related to the types found in corresponding blood, urine, and semen samples. DNase I typing could, therefore, provide a novel discriminant characteristic in the forensic examination of sweat.

Author Information

Yasuda, T
Fukui Medical School, Maebashi, Gunma, Japan Gunma University School of Medicine, Gunma, Japan
Takeshita, H
Fukui Medical School, Maebashi, Gunma, Japan
Sawazaki, K
Fukui Medical School, Maebashi, Gunma, Japan Scientific Investigation Laboratory, Fukui Prefectural Police Headquarters, Fukui, Japan
Nadano, D
Fukui Medical School, Maebashi, Gunma, Japan
Iida, R
Fukui Medical School, Maebashi, Gunma, Japan
Miyahara, S
Fukui Medical School, Maebashi, Gunma, Japan
Kishi, K
Fukui Medical School, Maebashi, Gunma, Japan Gunma University School of Medicine, Gunma, Japan
Pages: 3
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Stock #: JFS14012J
ISSN: 0022-1198
DOI: 10.1520/JFS14012J