Journal Published Online: 01 September 1996
Volume 41, Issue 5

Identification and Quantitation of Source from Hemoglobin of Blood and Blood Mixtures by High Performance Liquid Chromatography

CODEN: JFSCAS

Abstract

The described technique offers a sensitive and reproducible method for inferring the source of over 50 different animal species from bloodstains and blood mixtures. Hemoglobins from each of the species were examined using reversed-phase high performance liquid chromatography (HPLC) in chromatographic times of less than 25 mins. The HPLC method complements and furthers current methodology for identification of species of origin. HPLC analysis is particularly well suited for the quantitative analysis of blood and blood mixtures and is applicable to species for which antisera are unavailable. The sensitivity of the method (hemoglobin amounts down to 1.2 µg) lends itself to the analysis of blood mixtures in which only a small percentage of the mixture represents blood from a given species. Such resolution and quantitation is applicable to wildlife forensic casework.

Author Information

Espinoza, EO
National Fish and Wildlife Forensics Laboratory, Ashland, OR
Kirms, MA
National Fish and Wildlife Forensics Laboratory, Ashland, OR
Filipek, MS
National Fish and Wildlife Forensics Laboratory, Ashland, OR
Pages: 8
Price: $25.00
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Stock #: JFS14002J
ISSN: 0022-1198
DOI: 10.1520/JFS14002J