Anticoagulant coumarin derivatives, bromadiolone, brodifacoum, warfarin, and coumatetralyl were analyzed simultaneously with a modified analytical method. The method includes purification by Sep-Pak cartridge and HPLC analysis using isocratic elution with methanol: 0.8% Acetic acid (8:2). The recoveries of coumarin derivatives from biological samples ranged from 96 to 99%. The concentrations of coumarin derivatives in intoxicated rats' organ were also determined with this analytical procedure.
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