Journal Published Online: 01 July 1996
Volume 41, Issue 4

Multiplex Amplification and Typing Procedure for the Loci D1S80 and Amelogenin

CODEN: JFSCAS

Abstract

A method has been developed that enables multiplex amplification and simultaneous typing of the loci D1S80 and amelogenin using discontinuous polyacrylamide gel electrophoresis and silver staining. The protocol is sensitive, simple, rapid, and relatively inexpensive. The results of the multiplex analysis of the D1S80 and amelogenin loci were comparable to those obtained when each locus was analyzed individually. A small validation study was undertaken to evaluate the forensic applicability of this multiplex system. The data demonstrate that DNA exposed to a variety of environmental insults yields reliable multiplex typing results.

Author Information

Budowle, B
Forensic Science Research Unit, Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico, Virginia
Koons, BW
Forensic Science Research Unit, Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico, Virginia
Errera, JD
Forensic Science Research Unit, Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico, Virginia
Pages: 4
Price: $25.00
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Details
Stock #: JFS13973J
ISSN: 0022-1198
DOI: 10.1520/JFS13973J