Journal Published Online: 01 May 1996
Volume 41, Issue 3

PCR-Based Human Leukocyte Antigen (HLA) DQα Typing of Blood Stained Light and Dark Blue Denim Fabric

CODEN: JFSCAS

Abstract

Obtaining typable PCR products from DNA purified from blood stained blue denim has been difficult since inhibitors of PCR in blue denim apparently co-purify with the DNA. Organic and chelex extraction methods were tested for their ability to purify typable DNA from either light or dark blue denim fabric both stained with blood. DNA purified from the light blue denim using either method was successfully used in obtaining correct HLA-DQα typing results. The chelex, but not the organic, procedure was able to yield typable DNA when the dark blue denim was the substrate. Therefore, the chelex method may be more effective than the organic method in preventing compounds that inhibit PCR from co-purifying with the DNA.

Author Information

Del Rio, SA
Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD
Marino, MA
Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD
Belgrader, P
Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD
Pages: 3
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS13941J
ISSN: 0022-1198
DOI: 10.1520/JFS13941J