Journal Published Online: 01 September 1999
Volume 44, Issue 5

Potassium Nitrite Reaction with 11-Nor-Delta9-Tetrahydrocannabinol-9-Carboxylic Acid in Urine in Relation to the Drug Screening Analysis

CODEN: JFSCAS

Abstract

Recently potassium nitrite has been used as an adulterant to interfere with the analysis of 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) in urine. A comprehensive study of the THC-COOH and nitrite reaction chemistry and stability under various conditions is presented. Reverse phase high performance liquid chromatography (HPLC) and negative electrospray mass spectrometry (ESMS) results are given to substantiate the derived reaction mechanism and properties leading to reaction termination. The addition of potassium carbonate as a buffering agent prior to or following sample void as a means of preventing the formation of a nitroso-complexed form of the 11-nor-delta9-tetrahydrocannabinol-9-carboxylic acid is evaluated.

Author Information

Lewis, SA
Analytical Services Organization, Lockheed Martin Energy Systems, Inc., Oak Ridge, TN
Lewis, LA
Analytical Services Organization, Lockheed Martin Energy Systems, Inc., Oak Ridge, TN
Tuinman, A
University of Tennessee, Knoxville
Pages: 5
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS12022J
ISSN: 0022-1198
DOI: 10.1520/JFS12022J