Standard Active Last Updated: Nov 19, 2019 Track Document
ASTM E1951-14(2019)

Standard Guide for Calibrating Reticles and Light Microscope Magnifications

Standard Guide for Calibrating Reticles and Light Microscope Magnifications E1951-14R19 ASTM|E1951-14R19|en-US Standard Guide for Calibrating Reticles and Light Microscope Magnifications Standard new BOS Vol. 03.01 Committee E04
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Significance and Use

4.1 These methods can be used to determine magnifications as viewed through the eyepieces of light microscopes.

4.2 These methods can be used to calibrate microscope magnifications for photography, video systems, and projection stations.

4.3 Reticles may be calibrated as independent articles and as components of a microscope system.


1.1 This guide covers methods for calculating and calibrating microscope magnifications, photographic magnifications, video monitor magnifications, grain size comparison reticles, and other measuring reticles. Reflected light microscopes are used to characterize material microstructures. Many materials engineering decisions may be based on qualitative and quantitative analyses of a microstructure. It is essential that microscope magnifications and reticle dimensions be accurate.

1.2 The calibration using these methods is only as precise as the measuring devices used. It is recommended that the stage micrometer or scale used in the calibration should be traceable to the National Institute of Standards and Technology (NIST) or a similar organization.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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Book of Standards Volume: 03.01
Developed by Subcommittee: E04.03
Pages: 7
DOI: 10.1520/E1951-14R19
ICS Code: 37.020