Standard Active Last Updated: May 17, 2024 Track Document
ASTM E1721-24

Standard Test Method for Determination of Acid-Insoluble Residue in Biomass

Standard Test Method for Determination of Acid-Insoluble Residue in Biomass E1721-24 ASTM|E1721-24|en-US Standard Test Method for Determination of Acid-Insoluble Residue in Biomass Standard new BOS Vol. 05.06 Committee E48
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Significance and Use

4.1 The acid-insoluble residue content is used in conjunction with other assays to determine the total composition of biomass samples.


1.1 This test method covers determination of the acid-insoluble residue of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid and alkaline pretreated biomass, and the solid fraction of fermentation residues. All results are reported relative to the 105 °C oven-dried weight of the sample.

1.2 The residue collected contains the acid-insoluble lignin and any condensed proteins from the original sample. An independent nitrogen analysis would be required to determine the acid-insoluble lignin content separate from the condensed protein fraction and is outside the scope of this test method.

1.3 A portion of the lignin in some biomass samples will remain soluble during this procedure. The total lignin in a biomass sample includes both acid-soluble lignin and lignin in the acid insoluble residue.

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 8 and Note 3 and Note 5.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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Book of Standards Volume: 05.06
Developed by Subcommittee: E48.05
Pages: 4
DOI: 10.1520/E1721-24
ICS Code: 65.040.20