Standard Historical
Active: ASTM E1052-20
Last Updated: Jul 13, 2011
ASTM E1052-96(2002)

Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension (Withdrawn 2011)

Significance and Use

This test method is to be used to determine the effectiveness of antimicrobial solutions against designated prototype viruses that are in suspension.

The effective antimicrobial concentration should be determined using cell cultures as the host system for specific viruses.

This suspension test is for special applications of virucides, such as inactivation of viruses in contaminated liquid wastes, and as a first stage in determining virucidal potential of liquid chemical germicides, liquid hand soaps, OTC topicals or other skin products. Regulatory agencies may require additional tests to demonstrate overall virucidal activity.


1.1 This laboratory test method is a suspension test used to evaluate the effectiveness of antimicrobial solutions against specific viruses. This test method may be employed with most viruses and is designed for cell culture host systems.

1.2 This test method should be performed only by those trained in microbiological or virological techniques.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. The user should consult a reference for the laboratory safety recommendations.

1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practice regulations (GLPs) are required and to follow them where appropriate (40 CFR, Part 160 for EPA submissions and CFR, Part 58 for FDA submissions). Refer to the appropriate regulatory agency for performance standards of virucidal efficacy.

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Book of Standards Volume: 11.08
Developed by Subcommittee: E35.15
Pages: 3
DOI: 10.1520/E1052-96R02
ICS Code: 65.100.01