Standard Historical Last Updated: Jun 29, 2016 Track Document
ASTM D5465-93(2012)

Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods D5465-93R12 ASTM|D5465-93R12|en-US Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods Standard new BOS Vol. 11.02 Committee D19
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Significance and Use

These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.

Section
ACounting Colonies on Membrane Filters4
BCounting Colonies on Pour Plates5
CCounting Colonies on Spread Plates6

The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.

Scope

1.1 These practices cover recommended procedures for counting colonies and reporting colony-forming units (CFU) on membrane filters (MF) and standard pour and spread plates.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Details
Book of Standards Volume: 11.02
Developed by Subcommittee: D19.24
Pages: 4
DOI: 10.1520/D5465-93R12
ICS Code: 07.100.20