Standard Active Last Updated: Apr 19, 2019 Track Document
ASTM D4994-19

Standard Practice for Recovery of Viruses from Wastewater Sludges

Standard Practice for Recovery of Viruses from Wastewater Sludges D4994-19 ASTM|D4994-19|en-US Standard Practice for Recovery of Viruses from Wastewater Sludges Standard new BOS Vol. 11.02 Committee D19
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Significance and Use

4.1 Although many laboratories are presently isolating viruses from sludge, a valid comparison of data generated has not been possible because of the lack of a standard test method(s).


1.1 This practice is used for the recovery of viruses from wastewater sludges and favors the enteroviruses.

1.2 Both procedures are applicable to raw, digested, and dewatered sludges.





Procedure A—Adsorption

7 to 10

Procedure B—Sonication

11 to 15

1.3 This practice was tested on standardized sludges as described in 10.1. It is the user's responsibility to ensure the validity of this practice for untested matrices.

1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.6 Only adequately trained personnel should be allowed to perform these procedures and should use safety precautions recommended by the U.S. Public Health Service, Center for Disease Control,2 for work with potentially hazardous biological organisms.

1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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Book of Standards Volume: 11.02
Developed by Subcommittee: D19.24
Pages: 6
DOI: 10.1520/D4994-19
ICS Code: 13.060.30