Standard Historical Last Updated: Oct 15, 2012
ASTM D4133-82(2004)

Standard Practice for Sampling Phytoplankton with Pumps

Significance and Use

The advantages of a pumping phytoplankton sampler are as follows:

4.1.1 Sample size is more accurately controlled than with the use of a conical tow net.

4.1.2 Discrete samples from any depth are easily obtained.

4.1.3 Multiple or replicate samples are easily obtained.

4.1.4 They are adaptable to shallow ecosystems.

4.1.5 They allow for a stable collecting efficiency.

The disadvantages of a pumping phytoplankton sampler are as follows:

4.2.1 They generally are bulky and frequently require an electrical source.

4.2.2 They generally are costly.

4.2.3 They collect only qualitative samples, or semiquantitative samples when used with a volume register.

There are several special considerations that shall be observed when using a pumping phytoplankton sampler. They are as follows:

4.3.1 Pumps can induce mortality of the organisms and damage the delicate forms.

4.3.2 If tubing is not properly cleaned after use, contamination of subsequent samples can ensue.


1.1 This practice covers the procedures for obtaining qualitative samples of a phytoplankton community by use of pumping systems.

1.2 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Book of Standards Volume: 11.06
Developed by Subcommittee: E47.01
Pages: 2
DOI: 10.1520/D4133-82R04