Standard Active Last Updated: Feb 25, 2022
ASTM D3863-22

Standard Test Method for Retention Characteristics of 0.40 to 0.45 μm Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality 

Significance and Use

5.1 Microbiological water testing procedures using membrane filtration are based on the premise that all bacteria within a specific size range will be retained by the membrane filter used. If the membrane filter does not retain these bacteria, false negative results or lowered density estimates may occur that could have serious repercussions due to the presence of unrecognized potential health hazards in the water being tested, especially in drinking water.

5.2 This procedure as devised will enable the user to test each membrane filter lot number for its ability to retain all bacteria equal to, or larger than, the stated membrane pore size.


1.1 This test method covers a procedure to test membrane filters for their ability to retain bacteria whose diameter is equal to or slightly larger than membrane filters with pore size rated at 0.40 to 0.45 μm.

1.2 The procedures described are for the use of user laboratories as differentiated from manufacturers’ laboratories.

1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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Book of Standards Volume: 11.02
Developed by Subcommittee: D19.08
Pages: 4
DOI: 10.1520/D3863-22
ICS Code: 07.100.20; 13.060.70