ASTM WK28565

    New Practice for the Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems

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    Developed by Subcommittee: D22.08 | Committee D22 | Contact Staff Manager


    1. Scope

    1.1 The purpose of this standard practice is to describe procedures for the collection of culturable airborne fungal spores and/or fragments or bacteria on agar plates using inertial impaction sampling techniques. 1.2 This practice does not include collection of culturable fungi or bacteria by devices not using agar plates. 1.3 This standard practice presumes that the user has a fundamental understanding of field investigative techniques related to the scientific process, and sampling plan development and implementation. It is important to establish the related hypothesis to be tested and the supporting analytical methodology needed in order to identify the sampling media to be used and the laboratory conditions for analysis. 1.4 This standard practice does not address the development of a formal hypothesis or the establishment of appropriate and defensible investigation and sampling objectives. It is presumed the investigator has the experience and knowledge base to address these issues. 1.5 This standard practice does not provide the user sufficient information to allow for interpretation of the analytical results from sample collection. It is the users responsibility to seek or obtain the information and knowledge necessary to interpret the sample results reported by the laboratory. 1.6 The values stated in SI units are to be regarded as standard. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and to determine the applicability of regulatory limitations prior to use.


    Agar Plate; Bioaerosol; Calibration; Collection Efficiency; Colonies; Colony Forming Units; Culturable Fungi; Fungi; Fungus; Hyphae; Impactor; Indoor Air Quality; Inertial Impaction Sampler; Mold; Mould; Sampling; Spore


    This standard practice is intended for the collection of airborne particles on agar plates using inertial impaction for the purpose of culturing fungi. This standard practice is valuable when species level identification and/or quantity of culturable aerosolized fungi are important factors for the indoor air quality investigation. This practice can also be used to collect air samples for culturing bacteria. This standard is intended to provide indoor environment investigators with a basic understanding of the equipment, materials and instructions necessary to effectively collect air samples on agar plates using an inertial impactor. This standard is intended to minimize systematic sampling variations between different data sets.

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    Work Item Status

    Date Initiated:

    Technical Contact:
    John Neville