ASTM E3226 - 19

    Standard Test Method for Processing Cellulose Sponge-wipes to Detect Bacillus anthracis Spores Sampled from Environmental Surfaces

    Active Standard ASTM E3226 | Developed by Subcommittee: E35.15

    Book of Standards Volume: 11.08


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    Significance and Use

    5.1 This procedure describes a standardized method of processing cellulose wipes in a biosafety level 3 laboratory in order to detect and provide a semi-quantitative estimate of B. anthracis contamination after sampling of non-porous surfaces. Sampling may be conducted to characterize the extent of contamination or for clearance of an area after decontamination.

    1. Scope

    1.1 This test method covers a standardized method of processing cellulose wipes in a biosafety level 3 (BSL3) laboratory in order to detect and provide a semi-quantitative estimate of Bacillus anthracis contamination after sampling of non-porous surfaces. Sampling may be conducted to characterize the extent of the contamination, or for area clearance after decontamination.

    1.2 The laboratory procedures should be performed in a BSL3 laboratory by those trained for BSL3 microbiological techniques.

    1.3 This test method is specific to B. anthracis, but could be adapted for use with other organisms.

    1.4 The interlaboratory study was conducted with cellulose sponge wipes pre-moistened with neutralizing buffer. All reproducibility, sensitivity, and specificity data are based on the performance of these wipes. A review was conducted by subcommittee in 2019, and re-confirmed these ILS data are valid.

    1.5 Units—The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard.

    1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

    1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods

    E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method

    E2756 Terminology Relating to Antimicrobial and Antiviral Agents


    ICS Code

    ICS Number Code 07.100.01 (Microbiology in general); 71.040.20 (Laboratory ware and related apparatus)

    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/E3226-19

    Citation Format

    ASTM E3226-19, Standard Test Method for Processing Cellulose Sponge-wipes to Detect Bacillus anthracis Spores Sampled from Environmental Surfaces, ASTM International, West Conshohocken, PA, 2019, www.astm.org

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