ASTM E3152 - 18

    Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents

    Active Standard ASTM E3152 | Developed by Subcommittee: E35.15

    Book of Standards Volume: 11.08


      Format Pages Price  
    PDF 11 $58.00   ADD TO CART
    Hardcopy (shipping and handling) 11 $58.00   ADD TO CART



    Significance and Use

    3.1 Fungi are known to produce objectionable odors, stains, and premature biodeterioration of various consumer products and construction substrates including textiles, carpet, ceiling tile, gypsum wallboard, lumber, and plasticized vinyl and other polymers.

    3.2 Antifungal activity is typically:

    3.2.1 Determination of article susceptibility to fungal colonization,

    3.2.2 Determination of fungistatic activity (qualitative determination of prevented or delayed fungal colonization), and

    3.2.3 Determination of fungicidal/sporicidal activity (quantitative determination of spore kill).

    3.3 The degree of required surface examination varies from gross visual examination to detailed microscopic assessment among these methods.

    3.4 This guide provides an overview of established methods and suggestions for their applicability, with consideration to the type of substrate treated or the type of antifungal treatment being assessed.

    1. Scope

    1.1 This guide provides information on various test methods currently available to assess antifungal activity on natural or synthetic substrates.

    1.2 Knowledge of microbiological techniques is required for the practice of this guide.

    1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

    1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    C1338 Test Method for Determining Fungi Resistance of Insulation Materials and Facings

    D2020 Test Methods for Mildew (Fungus) Resistance of Paper and Paperboard

    D3273 Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber

    D3456 Practice for Determining by Exterior Exposure Tests the Susceptibility of Paint Films to Microbiological Attack

    D4141/D4141M Practice for Conducting Black Box and Solar Concentrating Exposures of Coatings

    D4300 Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi

    D4445 Test Method for Fungicides for Controlling Sapstain and Mold on Unseasoned Lumber (Laboratory Method)

    D4576 Test Method for Mold Growth Resistance of Wet Blue and Wet White

    D4783 Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi

    D5259 Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure

    D5590 Test Method for Determining the Resistance of Paint Films and Related Coatings to Fungal Defacement by Accelerated Four-Week Agar Plate Assay

    D6329 Guide for Developing Methodology for Evaluating the Ability of Indoor Materials to Support Microbial Growth Using Static Environmental Chambers

    D6469 Guide for Microbial Contamination in Fuels and Fuel Systems

    D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid FuelsFiltration and Culture Procedures

    D7436 Classification System for Unfilled Polyethylene Plastics Molding and Extrusion Materials with a Fractional Melt Index Using ISO Protocol and Methodology

    D7855/D7855M Test Method for Determination of Mold Growth on Coated Building Products Designed for Interior Applications Using an Environmental Chamber and Indirect Inoculation

    D7910 Practice for Collection of Fungal Material From Surfaces by Tape Lift

    E1326 Guide for Evaluating Non-culture Microbiological Tests

    E2111 Quantitative Carrier Test Method to Evaluate the Bactericidal, Fungicidal, Mycobactericidal, and Sporicidal Potencies of Liquid Chemicals

    E2197 Quantitative Disk Carrier Test Method for Determining Bactericidal, Virucidal, Fungicidal, Mycobactericidal, and Sporicidal Activities of Chemicals

    E2471 Test Method for Using Seeded-Agar for the Screening Assessment of Antimicrobial Activity In Carpets

    E2722 Test Method for Using Seeded-Agar for the Screening Assessment of Antimicrobial Activity in Fabric and Air Filter Media

    F1094 Test Methods for Microbiological Monitoring of Water Used for Processing Electron and Microelectronic Devices by Direct Pressure Tap Sampling Valve and by the Presterilized Plastic Bag Method

    G21 Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi

    ISO Standards

    ISO 16000 Indoor Air Sampling Strategy for Moulds

    ISO 16256 Clinical Laboratory Testing and in-vitro diagnostic test systems Reference method for testing the in vitro activity of antimicrobial agents against yeast fungi involved in infectious disease

    ISO 846 Evaluation of the Action of Microorganisms on Plastics

    JIS Standards

    JIS K 1571:2010

    BSi Standards

    BS 3900:Part G6:1989 British Standard Methods of test for paints Part G6. Assessment of resistance to fungal growth

    BS EN 113:1997

    AATCC Standards

    AATCC 174 (Part III)-2016 Antimicrobial Activity Assessment of Carpets

    AATCC 30-2015

    AWPA Standards

    AWPA E10-11 Standard Method of Testing Wood Preservatives by Laboratory Soil-Block Cultures


    ICS Code

    ICS Number Code 11.100.20 (Biological evaluation of medical devices)

    UNSPSC Code

    UNSPSC Code


    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/E3152-18

    Citation Format

    ASTM E3152-18, Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents, ASTM International, West Conshohocken, PA, 2018, www.astm.org

    Back to Top