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Significance and Use
5.1 Microbial decontamination of environmental surfaces by wiping is subject to many variables (, and failure to standardize them properly during testing of towelettes may give inconsistent test data. (See Practice ) and Test Method .) In particular, precise control of the pressure applied during wiping, the normally brief wiping times of a few seconds as well as the style and number of wiping strokes are difficult without a programmable mechanical device. The Wiperator has been designed and tested with these crucial factors in mind. The method described here is to assess the role of wiping in ridding non-porous environmental surfaces of bacterial contamination using prewetted towelettes, and also to determine if the used towelette can transfer viable contamination to clean surfaces on contact.
1.1 This standard is designed for use with a mechanized device (the Wiperator; ) to test pre-wetted towelettes.
1.2 Two species of vegetative bacteria, one Gram-positive coccus (Staphylococcus aureus) and one Gram-negative bacillus (Acinetobacter baumannii), representing important nosocomial pathogens, are used to separately contaminate disks of magnetized and brushed stainless steel in order to test the towelettes for their relative ability to:
1.2.1 Decontaminate non-porous environmental surfaces experimentally-contaminated with vegetative bacteria; and
1.2.2 Transfer any acquired bacterial contamination on the towelettes to clean surfaces.
1.3 This test method is not meant for use with towelettes for decontamination of skin.
1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses, if any, are for information only.
1.5 This test method should be performed by persons with training in microbiology in facilities designed and equipped for work with infectious agents at the appropriate biosafety level.
1.6 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) regulations are required and to follow them where appropriate.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
E2197 Quantitative Disk Carrier Test Method for Determining Bactericidal, Virucidal, Fungicidal, Mycobactericidal, and Sporicidal Activities of Chemicals
E2362 Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
E2896 Test Method for Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes
CFR Standards21 CFR 58 40 CFR 160 Good Laboratory Practice Standards
Other ReferenceOrganization for Economic Cooperation and Developm Guidance Document on Quantitative Methods for Evaluating the Activity of Microbicides used on Hard, Non-Porous Surfaces Available from OECD Environment Directorate, Environment, Health -Pascal 75775, Cedex 16, Paris, France (www.oecd.org/ehs/).
ICS Number Code 07.100.99 (Other standards related to microbiology); 71.100.35 (Chemicals for industrial and domestic desinfection purposes)
UNSPSC Code 42281912(Sterilization towels)
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ASTM E2967-15, Standard Test Method for Assessing the Ability of Pre-wetted Towelettes to Remove and Transfer Bacterial Contamination on Hard, Non-Porous Environmental Surfaces Using the Wiperator, ASTM International, West Conshohocken, PA, 2015, www.astm.orgBack to Top