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This test method covers the determination of isoaspartic acid residues in a protein or peptide sample.
Formerly under the jurisdiction of Committee E55 on Manufacture of Pharmaceutical Products, this test method was withdrawn in July 2012 in accordance with section 10.5.3.1 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.
1.1 This test method covers the determination of isoaspartic acid residues in a protein or peptide sample. This test method is applicable for the determination of isoaspartic acid residues in a sample in the range of 2.5-50 μmol/L. Higher concentrations can be determined following dilution. The reported lower range is based on single-operator precision.
1.2 The values stated in SI units are to be regarded as the standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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ASTM E1759-95(2003), Standard Test Method for Isoaspartic Acid in Proteins: Method for the Determination of Asparagine Deamidation Products (Withdrawn 2003), ASTM International, West Conshohocken, PA, 1995, www.astm.orgBack to Top